EXPRESSION PLASMID VECTOR FOR HETEROLOGICAL EXPRESSION OF RECOMBINANT PROTEINS, HIGH-FREQUENCY INTEGRATION AND AMPLIFIED AMPLIFICATION OF EXPRESSION CASSETTE IN CELLS OF MAMMALS, BICISTRONIC MESSENGER RIZIFORM, METHOD OF PRODUCTION OF STABLE LINES OF PRODUCENTS OF RECOMBINANT PROTEINS USING SPECIFIED VECTOR, METHOD FOR PRODUCTION OF RECOMBINANT PROTEINS Russian patent published in 2013 - IPC C12N15/63 C12N15/12 C12N5/10 

FIELD: biotechnologies.

SUBSTANCE: expression plasmid vector is described for heterologous expression of recombinant proteins, high-frequency integration and accelerated amplification of an expression cassette in cells of mammals. The vector comprises functional promotor and terminator of a gene of elongation factor 1 alpha of a Chinese hamster, flanked by 5' and 3' HTO of this gene; a section of cloning of open frames of reading target proteins; an internal ribosome entry site (IRES) of a encephalomyocarditis virus (EMCV); an open frame of reading of dihydrofolate reductase (DHFR) of a mouse, expressing within a bicistronic messenger riziform together the target gene (IRES DHFR); and a section of Epstein-Barr virus terminal repetition (EBVTR). Also the method is described to produce stable lines of recombinant protein producents with high representation of an expression cassette in a genome with usage of the specified expression plasmid vector.

EFFECT: method improvement.

12 cl, 10 dwg, 3 tbl, 11 ex