FIELD: biochemistry.
SUBSTANCE: disclosed is a method for automatic maintenance of concentration of dissolved gases in a culture medium, located in cell with a cell model and circulating via microfluid system channels, and device for realising said method. Method is based on use of proportional-integral-differential control. Measured parameter used is concentration of oxygen and/or carbon dioxide in layer of gas-permeable material in a separate measurement cell. Specified parameter used is a parameter calculated from required parameter of gas concentration in cell with cell model based on coefficient of communication of concentrations in measurement and cellular cells.
EFFECT: inventions provide absence of mutual negative influence of cell cultures and elements of device, longer service life of device, reliability of device with sufficiently high accuracy of maintaining concentration of dissolved gases and maintaining typically low for micro-fluid devices volume of liquid in channels.
10 cl, 6 dwg
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| PICKUP OF DISSOLVED OXYGEN | 0 |
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