METHOD AND MICROFLUIDIC CHIP FOR CELL CULTURE OR CELL MODEL Russian patent published in 2017 - IPC C12M3/00 C12N5/02 

FIELD: biotechnology.

SUBSTANCE: microfluidic chip and method for cells culturing or cell model in this microfluidic chip are proposed. The microfluidic chip includes microfluidic channels with valves to overlap the channels, a compartment for culturing, a working chamber with a membrane and a means of creating a constant pressure in the closed circuit. At that, the membrane is adapted to change the volume of working chamber. The working chamber, the valves and the compartment inlet are connected by microfluidic channels to form a closed loop of nutrient medium circulation. The working chamber with valves and microfluidic channels is a routing pump. The method comprises filling of microfluidic channels and compartment with nutrient medium, insertion of cells or a cell model into the culturing compartment. At that, constant pressure and pulsing flow of the nutrient medium are created in the circuit.

EFFECT: possibility of microcirculation modeling hydrodynamic component in vivo in a microfluidic, as well as organism systemic response simulation.

39 cl, 15 dwg