FIELD: medicine.
SUBSTANCE: CD4+- bone marrow cells are cultured in CO2-incubator at 37°C, 5% CO2 and 100% air humidity for 24 hours. Incubation is carried out in the culture medium of the following composition: 90% of RPMI-1640 medium, 10% of ETS, 280 mg/l of L-glutamine, 50 mg/l of gentamicin, 50 mcM of phosphatidylinositol-3-kinase inhibitor (PI3K).
EFFECT: increased production of erythropoietin.
1 tbl, 1 ex
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