FIELD: biotechnology.
SUBSTANCE: invention refers to biotechnology, namely to stimulation of erythropoietin production in bone marrow cells in vitro. Method involves culturing a fraction of bone marrow myelocarocytes in CO2-incubator at 37 °C, 5 % CO2 and 100 % air humidity for 24 hours in a complete culture medium of the following composition: 90 % of RPMI-1640 medium, 10 % ETS, 280 mg/l of L-glutamine, 50 mg/l of gentamycin with addition of JNK inhibitor in effective concentration.
EFFECT: invention enables higher erythropoietin production.
1 cl, 1 tbl, 1 ex
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