METHOD OF PRODUCING BACTERIAL PREPARATION WITH PROBIOTIC ACTIVITY Russian patent published in 2016 - IPC C12N1/20 A61K35/744 A61K35/745 A61K35/747 

FIELD: microbiology.

SUBSTANCE: invention relates to microbiology and can be used for manufacturing of functional food products. Method of producing bacterial preparation with probiotic activity based on species of normal human intestinal microflora, involving extraction of bifidobacteria and lactobacilli from human excrements, their cultivation on liquid nutrient medium in anaerobic conditions at temperature 37 °C with subsequent centrifugation and separation of bacterial preparation. Excrements of adult healthy subject are used, who does not take antibiotic or probiotic preparations for 3 months before extraction, streptococci and enterococci are additionally extracted from excrements. Culturing is carried out on medium containing (g/l): yeast extract - 5.0; papain overcook of soya flour - 10.0; sodium hydrophosphate - 1.0; Tween-80 - 0.2; ammonium citrate - 1.0; cysteine hydrochloride - 0.5; magnesium chloride - 0.5; ascorbic acid - 0.5; glucose - 10.0; meat extract - 20.0; manganese sulphate - 0.05; sodium azide - 0.2. Or cultivation can be performed on medium containing (g/l): proteose peptone - 15.0; papain overcook of soya flour - 10.0; sodium hydrophosphate - 1.5; Tween-80 - 1.0; ammonium citrate - 2.0; cysteine hydrochloride - 1.0; magnesium chloride - 0.5; ascorbic acid - 0.2; sodium chloride - 2.5; glucose - 10.0; meat extract - 10.0; sodium azide - 0.2. Or cultivation can be performed on medium containing (g/l): yeast extract - 10.0; pancreatic hydrolysate of casein - 20.0; sodium hydrophosphate - 2.5; Tween-80 - 0.7; lactose - 5.0; ammonium citrate - 0.5; cysteine hydrochloride - 1.0; magnesium chloride - 1.0; ascorbic acid - 0.1; sodium acetate - 0.2; sodium chloride - 3.0; glucose - 5.0; meat extract - 15.0; manganese sulphate - 0.1; sodium azide - 0.2. Cultivation is performed till concentration of microorganisms reaches 1.0·10⁸ COE/ml.

EFFECT: method enables to obtain probiotic preparation with antagonistic properties towards pathogenic and opportunistic pathogens, as well as antioxidant, hepatoprotective and anticancer activity.

1 cl, 2 tbl, 3 ex