FIELD: biochemistry.
SUBSTANCE: described is a group of inventions that includes a method for detecting two target nucleic acid sequences in a sample using different detection temperatures. In one embodiment of the invention, a method for detecting two target nucleic acid sequences in a sample includes a step of determining the presence of two target nucleic acid sequences based on signals, wherein the presence of a target nucleic acid sequence having a high detection temperature, determined on the basis of the signal detected at a high detection temperature, the presence of a target nucleic acid sequence having a low detection temperature is determined by the difference between the signal detected at a high detection temperature and the signal detected at a low detection temperature.
EFFECT: invention widens the range of methods for detecting nucleic acid sequences in a sample.
36 cl, 18 dwg, 5 ex
| Title |
Year |
Author |
Number |
| DETECTION OF NUCLEOTIDE TARGET SEQUENCES USING DIFFERENT DETECTION TEMPERATURES |
2014 |
- Chun Dzhon Joon
- Li Eng Cho
|
RU2780587C2 |
| DETECTION OF NUCLEOTIDE VARIATIONS IN NUCLEIC ACID SEQUENCE-TARGETS IN THE ANALYSIS WITH THE CLEAVAGE AND EXTENSION OF THE PROBING AND TAGGING OLIGONUCLEOTIDE (PTO) |
2013 |
- Chun Dzhon Joon
- Li Yang Dzho
|
RU2620955C2 |
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2012 |
- Chun Dzhon Joon
- Li Yang Dzho
|
RU2608501C2 |
| DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION ASSAY |
2012 |
- Chun Dzhon Joon
- Li Jang Dzho
|
RU2566562C2 |
| DETECTION OF NUCLEIC ACID TARGET SEQUENCE IN ANALYSIS WITHOUT HYBRIDIZATION, DEPENDING ON CLEAVAGE AND ELONGATION OF THE PROBING AND LABELING OLIGONUCLEOTIDE (PTO) |
2013 |
- Chun Dzhon Joon
- Li Yang Dzho
|
RU2620958C2 |
| DETECTION OF PLURALITY OF TARGET NUCLEIC ACIDS USING PLURALITY OF DETECTION TEMPERATURES |
2022 |
- Lee, Han Bit
- Kim, Jeong Woo
- Kim, Hyeon Be
|
RU2821888C2 |
| TARGET DETECTION WITH TSG-PRIMER |
2010 |
- Chun, Dzhon Joon
- Khvan, In Taehk
|
RU2551321C2 |
| METHOD OF DIAGNOSING LUPUS IN HUMAN |
2010 |
- Berens Timoti V.
- Grekhem Robert R.
|
RU2596391C2 |
| METHOD OF SPECIFIC IDENTIFICATION OF DNA SEQUENCES |
2017 |
- Vedernikov Vitalij Evgenevich
|
RU2665631C2 |
| TARGET-DISCRIMINATING PROBE, METHOD OF CONSTRUCTING THEREOF AND METHOD OF DETECTING NUCLEIC ACID TARGET-SEQUENCE (VERSIONS) |
2010 |
- Chun, Dzhon Joon
- Khvan, In Taehk
- Leeh, San Kil
|
RU2542478C2 |
