FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Described is a novel bispecific antibody construct, having a different specificity for each binding site and consisting of two copies of a polypeptide of one heavy chain and a first light chain and a second light chain, where first and second light chains are different. One of light chains contains a Kappa constant domain, and other contains a Lambda constant domain. Invention also describes methods of producing such construct. Described are methods of extracting antibodies of different specificity, but having a common heavy chain. Antibody according to invention enables to combine two targets simultaneously.
EFFECT: invention enables to minimise risk of immunogenicity.
36 cl, 22 dwg, 5 tbl, 15 ex
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