FIELD: chemistry.
SUBSTANCE: method for identifying products of DNA chemical hydrolysis involves chromatographic determination of hydrolysis products. The analysis is carried out using a chromatographic column filled with pentafluorophenyl-modified silicone dioxide, 4.6×150 mm, bead size 5 mkm, at 28°C. The adsorbed DNA hydrolysis products are eluted by a mixture of water, formic acid and acetonitrile in gradient mode as follows: during first stage gradient of acetonitrile varies linearly from 0 to 25% within 6 minutes; acetonitrile gradient of 25% is maintained for 4 minutes, the final stage involves phase equilibration for 4 minutes using 100% water with 0.1% formic acid, flow rate 0.6 ml/min.
EFFECT: provided reasonable separation of DNA chemical hydrolysis products for determination of DNA adducts.
2 cl, 2 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETERMINING CATECHOLAMINE DERIVATIVES IN URINE | 2018 |
|
RU2688184C1 |
| METHOD FOR CHROMATOGRAPHIC SEPARATION OF COMBINED SOLID DOSAGE FORM CONTAINING AMLODIPINE AND VALSARTAN | 2015 |
|
RU2603941C1 |
| METHOD OF SEMIQUANTITATIVE DETERMINATION OF TRITERPENE ACIDS IN SIBERIAN FIR TREE GREEN EXTRACTS BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY METHOD | 2023 |
|
RU2810636C1 |
| METHOD FOR MELDONIUM DETERMINATION IN HUMAN URINE | 2017 |
|
RU2639475C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF YESSOTOXINS IN MOLLUSCS | 2018 |
|
RU2716233C1 |
| METHOD OF HIGH-EFFICIENT LIQUID CHROMATOGRAPHIC DETERMINATION OF CONCENTRATIONS OF ALKYLPHOSPHONIC AND/OR O-ALKYLALKYLPHOSPHONIC ACIDS IN AQUEOUS SOLUTION | 2017 |
|
RU2653582C1 |
| METHOD FOR DETERMINING AMOUNT OF CARMOISINE IN JUICES | 2015 |
|
RU2596796C1 |
| METHOD OF QUANTITATIVE ANALYSIS OF URINE DIMETHYL TEREPHTHALATE BY LIQUID CHROMATOGRAPHY | 2010 |
|
RU2425380C1 |
| METHOD FOR DETERMINATION OF STEROID HORMONE DERIVATIVES IN URINE | 2021 |
|
RU2764363C1 |
| METHOD OF QUANTIFICATION OF LEVODOPA IN BLOOD PLASMA | 2017 |
|
RU2665164C1 |
