FIELD: biology.
SUBSTANCE: group of invention relates to molecular microbiology. Set of primers and method for detecting agent Acidovorax citrulli bacteria in seeds and vegetative parts of plants of Cucurbitaceae family is disclosed. Primers are specific to 16S ribosomal RNA and represent AC-1 F: 5'-ATGGTGTGTTCTTCGGGACC-3' (SEQ ID NO:A) and AC-1 R: 5'-ATCGGCGATCTCTTCGTGTC-3' (SEQ ID NO:B). To detect agent A. citrulli DNA is obtained from plant tissue. Obtained DNA is amplified with primers SEQ ID NO: A and SEQ ID NO: B. Initial denaturation is carried out at 96 °C for 5 minutes. Then one performs 35 cycles of denaturation at 96 °C for 5 s. Annealing is carried out at 63 °C for 30 s and elongation at 72 °C for 30 s. Final elongation is carried out at 72 °C for 5 minutes. Amplification product is detected by electrophoresis.
EFFECT: invention provides high sensitivity, enabling detection of A. citrulli in concentration up to 102 CFU/ml, as well as leading to reduced analysis time.
2 cl, 3 dwg, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETERMINING AMOUNT OF DAMAGE TO MITOCHONDRIAL DNA OF POTATOES WHEN TESTING TOXICITY OF PESTICIDES IN VITRO | 2020 |
|
RU2762904C1 |
| OLIGONUCLEOTIDE PRIMERS AND METHOD OF DETECTING DNA OF Mycobacterium paratuberculosis THAT IS PARATUBERCULOSIS AGENT BY METHOD OF POLYMERASE CHAIN REACTION (PCR) | 2010 |
|
RU2435852C1 |
| METHOD AND A SET OF PRIMERS FOR SPECIES-SPECIFIC IDENTIFICATION OF HUMAN BACTERIAL PNEUMONIA PATHOGENS | 2021 |
|
RU2781014C1 |
| SET FOR DETECTING LEGIONELLOSIS AGENT IN BIOLOGICAL MATERIAL AND ENVIRONMENTAL MEDIUM | 2009 |
|
RU2397251C1 |
| TEST SYSTEM AND METHOD FOR DETECTING SESN1 GENE DELETIONS | 2021 |
|
RU2772504C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBES AND METHOD FOR DETECTING DNA OF AGENTS OF GLANDERS AND MELIOIDOSIS BY PCR METHOD WITH PRODUCT DETECTION IN REAL TIME | 2019 |
|
RU2738358C1 |
| fr316 FRAGMENT OF MITOCHONDRIAL GENE ND1 INTENDED FOR OPISTORCHOSIS AGENTS DETECTION | 2006 |
|
RU2332456C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDES FOR IDENTIFYING NUCLEOTIDE SEQUENCE OF CODING PART OF NKX2,5, CFC1, GATA4 GENES AND RECOGNISING MUTATIONS ASSOCIATED WITH ORPHAN SINGLE-GENE PATHOLOGY UNDERLYING FAMILIAR CONGENITAL HEART DISEASE | 2013 |
|
RU2554056C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR DETECTING VARIANT STRAINS BURKHOLDERIA THAILANDENSIS, CONTAINING CLUSTER OF CAPSULAR POLYSACCHARIDE BIOSYNTHESIS GENES, HIGHLY HOMOLOGOUS BURKHOLDERIA PSEUDOMALLEI | 2019 |
|
RU2728356C1 |
| METHOD FOR DIAGNOSING HELICOBACTER PYLORI INFECTION AND ITS RESISTANCE TO CLARITHROMYCIN AND LEVOFLOXACIN | 2022 |
|
RU2806581C1 |
