FIELD: medicine.
SUBSTANCE: there are offered synthetic oligonucleotide primers having the following base composition: (SEQ ID NO: 5) gaagggtgttcggggccgtcgcttagg and (SEQ ID NO: 6) ggcgttgaggtcgatcgcccacgtgac and complementary for a genome IS900 region specific for M.paratuberculosis that is a paratuberculosis agent. There is offered a one-round method for detecting DNA of Mycobacterium paratuberculosis that is a paratuberculosis agent, assisted by oligonucleotide primers (SEQ ID NO: 5) gaagggtgttcggggccgtcgcttagg and (SEQ ID NO: 6) ggcgttgaggtcgatcgcccacgtgac by polymerase chain reaction (PCR). The method includes DNA recovery, DNA amplification on oligonucleotide primers, transfer of the amplification product on gel followed by result detection in a transilluminator; a positive reaction enables synthesising a fragment matched with size 413 bps.
EFFECT: invention enables instant diagnostics of paratuberculous infection.
3 cl, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
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| METHOD OF PREPARING ANTIGEN FOR SEROLOGICAL DIAGNOSIS OF PARATUBERCULOSIS IN RUMINANT ANIMALS | 1996 |
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| METHOD OF IDENTIFYING MYCOBACTERIA BY POLYMERASE CHAIN REACTION | 2010 |
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