TWO-LAYER, FLAT, TRANSPARENT HYDROGEL SUBSTRATE FOR LONG-TERM CELL CULTIVATION Russian patent published in 2017 - IPC C12M3/00 C12N5/07 C12N11/04 C08J5/18 

FIELD: biotechnology.

SUBSTANCE: two-layer, flat, transparent hydrogel substrate is proposed for long term cell cultivation and the method to get it. The substrate in the hydrated state has a thickness in the range of 25-500 microns, and the radius of menisci curvature of 10-150 microns. The hydrogel substrate includes a structure forming layer consisting of a three-dimensional collagen hydrogel and an upper protein layer. The three-dimensional hydrogel comprises collagen of type I, collagen of type III and proteins that promote cell adhesion and migration. The upper protein layer contains collagen of type IV, laminin and fibronectin. The method of substrate preparing comprises mixing of solutions of collagen of type I, collagen of type III and proteins that promote cell adhesion and migration and further gelation and vitrification, followed by proteins immobilization on the surface.

EFFECT: preservation of phenotype by endothelial cells and epithelial cells in their long-term cultivation.

13 cl, 1 dwg, 3 tbl, 8 ex