FIELD: biotechnology.
SUBSTANCE: synthetic DNA is prepared that encodes anti-mullerian human hormone, contains anti-mullerian human hormone gene with a codon composition and guanine and cytosine content, optimized for expression in Chinese hamster ovary cells. Based on the obtained DNA expression vector pTVK4pu/MISOPT is constructed, with scheme shown in Fig. 1, which contains a puromycin resistant gene as a selectable marker. pTVK4pu/MISOPT expression vector transforms CHO cells to produce a strain of Chinese hamster ovary cells CHO-MIS - recombinant anti-mullerian human hormone deposited under number RKKK(P) 775D. Strain CHO-MIS provides accumulation of up to 200 mg/l of AMG in the culture medium for 11 days in serum-free chemically defined medium with a composition containing no animal-derived components.
EFFECT: possibility of application for drug development.
3 cl, 7 dwg, 2 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MONOCLONAL ANTIBODY TO THE C- TERMINAL FRAGMENT OF ANTI -MULLER HORMONE | 2021 |
|
RU2770003C1 |
| MONOCLONAL ANTIBODY AGAINST THE C-TERMINAL FRAGMENT OF ANTI-MULLERIAN HORMONE | 2021 |
|
RU2764795C1 |
| HUMAN INTERLEUKIN-7 ENCODING SYNTHETIC DNA, EXPRESSION VECTOR CONTAINING THIS DNA (VERSIONS), HUMAN INTERLEUKIN-7 PRODUCING STRAIN, AND METHOD FOR HUMAN INTERLEUKIN-7 PRODUCTION | 2015 |
|
RU2615447C1 |
| MONOCLONAL ANTIBODIES TO C-END FRAGMENT OF ANTI-MULLER HORMONE | 2018 |
|
RU2765689C2 |
| METHOD OF PRODUCING HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE, PRODUCING CELL LINE AND PLASMID EXPRESSION VECTORS | 2019 |
|
RU2697273C1 |
| STRAIN OF CELLS OF CHINESE HAMSTER OVARIES - PRODUCER OF RECOMBINANT ANTIBODY AGAINST TUMOUR NECROSIS FACTOR ALPHA OF HUMAN BEING | 2014 |
|
RU2556816C1 |
| SYNTHETIC DNA ENCODING HUMAN ERYTHROPOIETIN, COMPRISING ITS VECTOR, METHOD OF PRODUCTION OF ERYTHROPOIETIN PRODUCER STRAIN, ERYTHROPOIETIN PRODUCER STRAIN | 2014 |
|
RU2548806C1 |
| EXPRESSION PLASMID FOR HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) IN CHO CELLS, EXPRESSION PLASMID FOR BETA-SUBUNIT OF HUMAN RECOMBINANT FSH IN CHO CELLS, CHO CELL PRODUCING HUMAN RECOMBINANT FSH, AND METHOD FOR PRODUCING ABOVE HORMONE | 2013 |
|
RU2560596C2 |
| HUMANIZED ANTIBODY TO CONFORMATIONAL C3 EPITOPE CONSTITUENT OF HUMAN COMPLEMENT, DNA SEQUENCE (VERSIONS), EXPRESSION VECTOR CONTAINING DNA SEQUENCE (VERSIONS), AND OVARIAN CELLS' STRAIN OF CHINESE HAMSTER CHO-HUMC34-PRODUCER OF THIS HUMANIZED ANTIBODY | 2016 |
|
RU2630647C1 |
| STRAIN OF CHINESE HAMSTER OVARY CELLS CHO-EPO 4A9 - PRODUCER OF HIGHLY SIALYLATED ERYTHROPOETHIN | 2016 |
|
RU2652884C1 |
