FIELD: biotechnology.
SUBSTANCE: group of inventions includes a method and a device for nucleic acids preparation from a biological sample and a method for nucleic acids amplification in a biological sample. The method for nucleic acids preparation comprises sample contacting with a substrate containing cellulose material. Thus, the cellulosic material is functionalized by biocidal agent. The biocidal agent comprises a quaternary ammonium compound and is able to weaken the cell membrane, cell wall, viral envelope, viral capsid or to lyse cellular or viral material. The device includes the above-mentioned substrate. The amplification method includes obtaining of nucleic acids using the above method and amplification of nucleic acid obtained.
EFFECT: inventions provide quick and easy preparation of nucleic acids from a biological sample for further processing.
18 cl, 11 dwg, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| COMPOSITIONS AND METHODS FOR DETECTION AND IDENTIFICATION OF NUCLEIC ACID SEQUENCES IN BIOLOGICAL SAMPLES | 2012 |
|
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|
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| MODULATION OF POLYMER GRANULES FOR DNA PROCESSING | 2019 |
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|
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| METHODS FOR ENCAPSULATING SINGLE CELLS, ENCAPSULATED CELLS, AND METHODS OF APPLICATION THEREOF | 2019 |
|
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|
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| METHOD OF PREPARING SAMPLE FOR DETERMINATION OF MICROORGANISMS (VARIANTS) AND SET FOR SAMPLE TREATMENT FOR DETERMINATION OF MICROORGANISMS | 1995 |
|
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| METHOD FOR AUTOMATED EXTRACTION WITH SIMULTANEOUS PURIFICATION OF NUCLEIC ACIDS FROM SEVERAL BIOLOGICAL SAMPLES | 2014 |
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| METHODS FOR DNA AMPLIFICATION TO PRESERVE METHYLATION STATUS | 2018 |
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