METHOD FOR BIOORESORABLE POLYMER CELL MATRIX FORMATION FOR TISSUE REGENERATION Russian patent published in 2017 - IPC A61F2/28 A61L27/14 A61L27/38 A61L27/44 A61L27/58 C12N5/02 

FIELD: pharmacology.

SUBSTANCE: invention is a method to form a bioresorbable polymeric cellular matrix for tissue regeneration, which consists in litographic production of a set of two-dimensional matrices in the form of a polymer film with superficial arrays of micro- and nanoobjects, which for each two-dimensional matrix are performed with individual architecture, systemicity and interconnection of micro- and nanoobjects location in the architecture, with a possibility of specifying the bone tissue structure to be formed, taking into account its biological functions, with a possibility of providing mechanical support, controlling the cell differentiation and proliferation processes, then the two-dimensional matrices are assembled into a supporting framework for cell cultures and biological agents, orienting them relative to each other with a possibility of specifying the bone tissue structure and fixing a stack, while difference is that the assembly is performed in liquid medium hardened to a bioresorbable material at photoexposure, two-dimensional matrix are sequentially set against each other with a gap, in which during successive installation layers of bioresorbable polymer are obtained by projection three-dimensional printing using a digital projector; the layers contain arrays of microscopic objects with individual architecture, system and interconnectivity of their location in the architecture, ability to specify the external form and internal three-dimensional matrix structure according to the three-dimensional computer model of the bone, with a possibility to provide mechanical support, cell proliferation and differentiation process control in the orthogonal direction relative to the surface of the two-dimensional matrices.

EFFECT: improved accuracy of structure imitation, of tissue being formed, achieved imitation accuracy in three dimensions, setting of the three-dimensional shape of matrix imitating the bone, based on a three-dimensional computer model of the bone that defines both the external shape and the internal three-dimensional microstructure.

11 cl, 3 dwg, 3 ex