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(19)

(11)

2 838 201

(13)

(51)

IPC

B82B3/00(2006-01-01)

B82Y5/00(2011-01-01)

B82Y40/00(2011-01-01)

C12N15/113(2010-01-01)

C12N15/12(2006-01-01)

C07K14/47(2006-01-01)

C08G73/02(2006-01-01)

A61K48/00(2006-01-01)

A61K47/34(2006-01-01)

A61P43/00(2006-01-01)

(21) (22)

Application

2021137790, 2021-12-20

(24)

Start date

2021-12-20

(22)

Actual filing date

2021-12-20

(45)

Published

2025-04-14

(72)

Inventor

Nikitin Maksim Petrovich

(73)

Holder

Federalnoe Gosudarstvennoe Avtonomnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Fiziko-Tekhnicheskij Institut Issledovatelskij Universitet)"

METHOD FOR SYNTHESIS OF TRANSFECTION NANOAGENT FOR GENE THERAPY APPLICATIONS (EMBODIMENTS) Russian patent published in 2025 - IPC B82B3/00 B82Y5/00 B82Y40/00 C12N15/113 C12N15/12 C07K14/47 C08G73/02 A61K48/00 A61K47/34 A61P43/00

Abstract RU 2838201 C1

FIELD: biotechnology.

SUBSTANCE: nanoparticles according to the present invention consist of protein molecules (immunoglobulin or transferrin) and polyethyleneimine (PEI); and include nucleic acids (NA) as a payload. Method of producing according to present invention includes steps of heating a solution containing nucleic acid, polyethyleneimine and protein, and subsequent short-term heating of the obtained mixture to a temperature in range of 45 °C to 99 °C. Invention can also be used in various fields of medicine when producing nanoforms of medicinal preparations.

EFFECT: invention discloses a method of producing transfection agents and gene therapeutic preparations.

29 cl, 3 dwg, 13 ex

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RU 2 838 201 C1

Authors

Nikitin Maksim Petrovich

Dates

2025-04-14—Published

2021-12-20—Filed