METHODS FOR IDENTIFICATION, SELECTION, ENRICHMENT AND DEPLETION OF Tr1 CELLS POPULATIONS, Tr1 CELLS POPULATIONS, PHARMACEUTICAL COMPOSITIONS, METHODS FOR THERAPY EFFECT MONITORING Russian patent published in 2017 - IPC G01N33/577 

FIELD: medicine.

SUBSTANCE: invention methods relate to identification, selection, enrichment, depletion of populations of relaxing Tr1 cells and/or activated Tr1 cells, as well as in vitro monitoring of the therapy effect. The pharmaceutical compositions of the invention contain cells expressing CD4, CD25, CD127 and CD62L markers. The kit of the invention comprises means to determine CD4, CD25, CD127 and CD62L markers expression on the cell surface. The use of the invention allows to identify the relaxing Tr1 cells as having a CD4⁺CD25^-CD127^lo/-CD62L^lo/- phenotype, and the activated Tr1 cells as having CD4⁺CD25⁺CD127^lo/-CD62L^lo/- phenotype.

EFFECT: ability to use to isolate, enrich or deplete the population of relaxing Tr1 cells or activated Tr1 cells.

15 cl, 2 tbl, 5 dwg, 4 ex