FIELD: molecular biology.
SUBSTANCE: invention relates to molecular biology. A method for extracting nucleic acids from nail plates is proposed, including lysis of biological material in a buffer with guanidine thiocyanate, incubation for 20 minutes at 65°C, mixing, centrifugation at 13,000 rpm for 2 minutes; then precipitation of the supernatant liquid with isopropanol, removal of the supernatant and washing of the precipitate with alcohol solutions, dissolution of the precipitate containing nucleic acids in the buffer for elution.
EFFECT: invention provides increased purity and quantity of the resulting preparation of nucleic acids.
5 cl, 2 dwg, 5 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING AND LABORATORY CONFIRMATION OF INHERITED CHROMOSOMALLY-INTEGRABLE HUMAN HERPES VIRUS 6A/B | 2020 |
|
RU2739997C1 |
| METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION | 2022 |
|
RU2802931C1 |
| METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND DNA OF HUMAN HERPES VIRUS 6B (HHV-6B) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION | 2022 |
|
RU2800995C1 |
| METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND DNA OF HUMAN HERPES VIRUS 6B (HHV-6B) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION | 2022 |
|
RU2800731C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR SPECIES DIFFERENTIATION OF HUMAN HERPES VIRUS 6A AND HUMAN HERPES VIRUS 6B AND METHOD OF ITS USE | 2022 |
|
RU2806427C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBE FOR DETECTION OF HUMAN HERPES VIRUS 6B | 2022 |
|
RU2806428C1 |
| METHOD FOR RECOVERY OF NUCLEIC ACIDS | 2014 |
|
RU2584346C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR THE DETECTION OF HUMAN HERPES VIRUS 6B | 2022 |
|
RU2802953C1 |
| METHOD FOR RNA AND DNA ISOLATION FROM DRY BIOLOGICAL SAMPLES STORED ON PAPER MEDIA AND SET FOR ITS IMPLEMENTATION | 2016 |
|
RU2628695C1 |
| METHOD OF DETERMINING LEVEL OF CHIMERIC GENE Trim5a EXPRESSION | 2015 |
|
RU2592675C1 |
