A METHOD FOR PRODUCING BASIC AMINO ACID (VARIANTS) Russian patent published in 2017 - IPC C12M1/04 C12M1/34 C12P13/08 C12P13/10 C12N1/20 

FIELD: biotechnology.

SUBSTANCE: method for producing a basic amino acid (variants) is proposed. The method comprises culturing a microorganism and collecting basic amino acid. The cultivation is carried out by controlling the concentration of ammonia in culture medium by using process and ammonia control device, wherein the device comprises an ammonia supply device, an ammonia sensor and a control part. In one embodiment, the ammonia concentration is controlled by control part and the concentration of non-ionized ammonia in the culture tank is calculated by substituting signal from ammonia sensor in calibration curve and instructing the device to supply ammonia to the culture tank. In another embodiment, the total concentration of ammonia in the medium is adjusted so that it is in a certain concentration range during at least part of the process. Thus, ammonia sensor reacts to non-ionized ammonia in culture medium, the control part creates a calibration curve, calculates the concentration of non-ionized ammonia of culture medium by substituting signal from ammonia sensor in the calibration curve and instructs ammonia supply device to supply ammonia to the culture tank.

EFFECT: invention provides the possibility of cultivation under continuous and arbitrary control of ammonia concentration in culture medium.

24 cl, 10 dwg, 2 tbl