FIELD: biotechnology.
SUBSTANCE: expression vector comprising an antibody light chain expression cassette, an antibody heavy chain expression cassette, and an expression marker cassette are proposed. The expression cassettes are arranged in a direction from 5'- to 3'-terminal of the sequence in the following order: antibody heavy chain expression cassette, antibody light chain expression cassette, and expression cassette of the selection marker. All three expression cassettes contain human elongation factor 1α promoter. Methods for eukaryotic cell transfection with the said vector and methods for production of an antibody using the obtained transfected cells are also disclosed.
EFFECT: invention allows to obtain a large number of highly productive clones producing antibodies.
30 cl, 10 dwg
