FIELD: biotechnology.
SUBSTANCE: methods include cell transfection of a mammal by expression vector, containing the first expression cassette, containing in the direction of 5'-3' hCMV promoter, nucleic acid encoding the light antibody chain, bGH poly (A)-signal sequence and a sequence of hGT terminator, and the second expression cassette, containing in the direction 5'-3' hCMV promoter, nucleic acid, encoding the heavy antibody chain, bGH poly (A)-signal sequence and hGT terminator sequence, where the first expression cassette and the second expression cassette are located in one direction, and where the expression vector additionally contains a selection marker, and production of many recombinant cells of a mammal with the subsequent selection of the many isolated recombinant cells of a mammal.
EFFECT: inventions make it possible to obtain stable antibodies.
27 cl, 15 dwg
