METHOD FOR DIAGNOSTICS OF INFECTIOUS DISEASE PERIOD CAUSED BY VARICELLA ZOSTER VIRUS IN CHILDREN Russian patent published in 2017 - IPC G01N33/53 

FIELD: biotechnology.

SUBSTANCE: test serum is simultaneously added to the wells of two strips immobilized with a varicella zoster virus antigen, incubated for 50-55 minutes, then one strip is treated once with an aqueous solution of 6 M of urea for 15 minutes, after which both strips are washed and conjugated with specific monoclonal antibodies to human IgG antibodies, incubated for 30 minutes, stained after washing with an aqueous chromogen solution for 10-15 minutes at room temperature, the reaction is stopped with a solution of 0.5 M of sulfuric acid, optical density is determined with a spectrophotometer, the avidity index is calculated using the formula: avidity index (AI) = (optical density of the sample treated with a solution of 6 M of urea/optical density of the sample without treatment with a solution of 6 M of Urea)X100%, and with an AI level less than 45% and up to 50% an acute period is diagnosed, with AI more than 50% and less than 65% - an early past infection, with AI of 65% and more than 70% - the late disease period is diagnosed.

EFFECT: method is effective and informative for diagnostics of the period of infectious disease caused by the varicella zoster virus in children, used to establish acute infection, activation of chronic persistent infection, and high protective immunity, which is decisive for determination of disease etiology and choice of adequate therapeutic tactics.

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