FIELD: biotechnology.
SUBSTANCE: invention relates to biochemistry, in particular, to a recombinant microorganism of Enterobacteriaceae bacteria family, such as E.coli optimized for the enzymatic production of methionine. The specified microorganism is modified in such a way that it has a weakened activity of cobalamin-independent MetE methionine synthase, and the metH gene is overexpressed compared to an unmodified microorganism. The invention also discloses a method of enzymatic production of methionine by cultivation of the specified recombinant microorganism in a culture medium containing carbon and sulfur source.
EFFECT: invention allows for increasing the methionine yield.
10 cl, 9 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MICROORGANISM FOR METHIONINE PRODUCTION WITH ENHANCED METHIONINE EFFLUX | 2014 |
|
RU2678757C2 |
| METHOD AND MICROORGANISM FOR ENZYMATIC PRODUCTION OF METHIONINE WITH IMPROVED OUTPUT OF METHIONINE | 2015 |
|
RU2723714C2 |
| METHOD FOR ENZYMATIC PRODUCTION OF SULFUR-CONTAINING AMINO ACIDS | 2012 |
|
RU2651511C2 |
| METHOD FOR FERMENTATIVE PRODUCTION OF L-AMINO ACIDS USING IMPROVED STRAINS OF ENTEROBACTERIACEAE FAMILY | 2013 |
|
RU2672614C2 |
| RECOMBINANT MICROORGANISMS PRODUCING METHIONINE | 2006 |
|
RU2447146C2 |
| MICROORGANISM FOR PRODUCING O-ACETYL-HOMOSERINE AND METHOD FOR PRODUCING O-ACETYL-HOMOSERINE USING SAME | 2015 |
|
RU2676137C2 |
| MICROORGANISM FOR PRODUCING O-ACETYLHOMOSERINE AND A METHOD OF PRODUCING O-ACETYLHOMOSERINE USING SAID MICROORGANISM | 2015 |
|
RU2710323C2 |
| MODIFIED POLYPEPTIDE HAVING HOMOSERINE ACETYLTRANSFERASE ACTIVITY AND MICROORGANISM EXPRESSING IT | 2011 |
|
RU2557411C2 |
| MICROORGANISM WITH ABILITY TO PRODUCE O-SUCCINYLHOMOSERINE OR AMBER ACID AND METHOD OF PRODUCING AMBER ACID OR O-SUCCINYLHOMOSERINE THEREWITH | 2015 |
|
RU2674891C2 |
| L-AMINO ACID PRODUCING MICROORGANISM AND METHOD FOR PRODUCING L-AMINO ACID WITH ITS USE | 2020 |
|
RU2795161C1 |
