FIELD: biotechnology.
SUBSTANCE: invention represents a recombinant strain of E.coli for enzymatic production of methionine, where in said recombinant strain expression of ygaZH genes, homologous to genes ygaZ and ygaH from Escherichia coli, is increased compared to the unmodified E.coli strain, provided that said recombinant strain does not have over expression of metH genes from E. coli and weakening expression of at least one gene among metN, metI or metQ, wherein said ygaZH-homologous genes are selected from a group of genes coding a pair of YgaZH-homologous genes defined in SEQ ID NO: 3 and SEQ ID NO: 4 from Citrobacter koseri, SEQ ID NO: 9 and SEQ ID NO: 10 from Enterobacter sp. (R4-368), SEQ ID NO: 17 and SEQ ID NO: 18 of Citrobacter youngae, SEQ ID NO: 19 and SEQ ID NO: 20 from Citrobacter freundii.
EFFECT: invention enables to obtain methionine with high efficiency.
9 cl, 11 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING L-METHIONINE | 2016 |
|
RU2729012C2 |
| NEW O-SUCCINYL HOMOSERINE TRANSFERASE VARIANT AND METHOD FOR SYNTHESIS OF O-SUCCINYL HOMOSERINE USING SAID VARIANT | 2018 |
|
RU2747493C1 |
| NEW O-SUCCINYL HOMOSERINE TRANSFERASE MUTANT AND METHOD FOR SYNTHESIS OF O-SUCCINYL HOMOSERINE USING SAID MUTANT | 2018 |
|
RU2747494C1 |
| MICROORGANISMS FOR PRODUCING ORNITHINE AND METHOD OF PRODUCING ORNITHINE USING SAID MICROORGANISMS | 2016 |
|
RU2721852C1 |
| MICROORGANISMS PRODUCING L-VALINE, AND METHOD OF PRODUCING L-VALINE USING SAME | 2021 |
|
RU2822660C1 |
| NEW VARIANT OF L-TYROSINE-EXPORTING PROTEIN AND METHOD OF OBTAINING L-TYROSINE USING IT | 2021 |
|
RU2808831C1 |
| VARIANT OF GLUTAMATE CYSTEINE LIGASE AND METHOD OF PRODUCING GLUTATHIONE USING SAME | 2021 |
|
RU2821273C1 |
| NEW VARIANT OF AN L-TRYPTOPHAN EXPORTING PROTEIN AND METHOD FOR PRODUCING L-TRYPTOPHAN WITH APPLICATION THEREOF | 2020 |
|
RU2763603C1 |
| BACTERIUM WHICH PRODUCES MONOPHOSPHORYL LIPID A, AND A METHOD OF PRODUCING MONOPHOSPHORYL LIPID USING A BACTERIUM | 2016 |
|
RU2727014C2 |
| INSECTICIDAL PROTEINS AND METHODS FOR USING THEM | 2016 |
|
RU2740312C2 |
