ANTI-POLY-UBIQUITINATED ANTIBODIES AND METHODS OF APPLICATION Russian patent published in 2017 - IPC C07K16/08 A61K39/395 

FIELD: biotechnology.

SUBSTANCE: isolated monoclonal antibody is described, that specifically binds to polyubiquitin containing a C-terminal linkage to the N-terminus, at that, this antibody does not bind specifically to monoubic quinine. A nucleic acid encoding the said antibody, an expression vector comprising the said acid and a host cell are described. An immunoconjugate comprising the said antibody and a cytotoxic agent, as well as a pharmaceutical formulation, are described. Application of the said antibody for the manufacture of a medicament for treatment of a disease or disorder associated with polyubiquitin activity is described. A method is provided for determination of polyubiquitin or polyubiquitinated protein presence in a sample where the polyubiquitin content is assumed, including sample exposure to at least one of the said antibodies and determination of binding of at least one antibody to the polyubiquitin or polyubiquitinated protein in the said sample. A method for polyubiquitinated protein separation is provided.

EFFECT: invention provides an antibody that specifically binds to polyubiquitin and does not bind to monoubicquitin.

49 cl, 38 dwg, 12 tbl, 5 ex