FIELD: biotechnology.
SUBSTANCE: method for separation of a protein of interest from the cell culture solution using two-stage ultrafiltration in tangential flow. The method includes the first operation using the device for ultrafiltration in tangential flow and the second operation using the device for ultrafiltration in tangential flow. During the first operation, the first cell culture supernatant flow is filtered using the first ultrafiltration membrane, during the second operation, the second cell culture supernatant flow is filtered using the second ultrafiltration membrane. At that, during the first operation, the membrane is chosen with such cutoff boundary, that the specified protein collected in the retentate, and during the second operation, the membrane is chosen with such cutoff boundary, that the specified protein collected in the permeate.
EFFECT: reduced level of pollutants and/or impurities in the feeding stream of the cell culture supernatant.
25 cl, 4 dwg, 4 tbl, 4 ex
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