FIELD: biotechnology.
SUBSTANCE: group of inventions relates to biotechnology. Proposed are the methods for creating a bank of high density cryopreserved mammal cells without using centrifugation (variants). Said methods involve the culturing of cells in a perfusion bioreactor coupled to a system of alternating tangential cross-flow filtration, non-centrifugally concentrating said cells using a filter or a system of alternating tangential cross-flow filtration and cryopreserving the concentrated cell population.
EFFECT: inventions provide the creation of a bank of high density viable cells with a low level of apoptosis.
17 cl, 15 dwg, 8 tbl, 6 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS FOR CREATION OF CELLS BANKS WITH ULTRAHIGH DENSITY | 2015 |
|
RU2723129C2 |
| METHODS OF CREATING ULTRAHIGH DENSITY CELL BANKS | 2015 |
|
RU2826038C2 |
| PERFUSION FILTRATION METHOD FOR CONTINUOUS CULTURING OF CELL CULTURES | 2022 |
|
RU2794425C1 |
| PERFUSION FILTRATION METHOD OF CONTINUOUS CULTURING OF CELL CULTURES | 2022 |
|
RU2800874C1 |
| CULTIVATION PROCESSES IN SEED FERMENTER SYSTEM AND THEIR APPLICATION METHODS | 2015 |
|
RU2786997C2 |
| METHOD OF CULTIVATION IN SYSTEM OF INOCULATING FERMENTORS (EMBODIMENTS) | 2015 |
|
RU2694327C2 |
| SMALL-SCALE METHOD OF CULTIVATION OF SUSPENDED CELLS | 2016 |
|
RU2730656C2 |
| METHOD FOR STEP-BY-STEP RETENTION AND PRODUCTION MODULE FOR OBTAINING BIOMACROMOLECULES AND THEIR APPLICATION | 2019 |
|
RU2769767C1 |
| METHOD OF PRODUCING AFLIBERCEPT | 2016 |
|
RU2732237C2 |
| ASSEMBLY UNITS OF PERFUSION BIOREACTOR BAGS | 2017 |
|
RU2755725C2 |
