FIELD: biotechnology.
SUBSTANCE: methods include addition of one or more recombinant polypeptides capable of catalyzing mogrol glycosylation to produce a mogroside compound having the amino acid sequence presented in SEQ ID NO: 1-5 or a cell lysate derived from a recombinant host expressing one or more of the said polypeptides, and mogrol of vegetable or synthetic origin into the reaction mixture, synthesis and isolation of the mogroside compound from the reaction mixture.
EFFECT: invention allows to obtain mogroside Ia, mogroside Ib or a mogroside compound glycosylated for C25-OH.
11 cl, 4 dwg, 4 ex
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Authors
Dates
2017-10-16—Published
2012-11-19—Filed