FIELD: biotechnology.
SUBSTANCE: bacterial host cell is provided for production of fucosylated oligosaccharides containing an expression vector that encodes a polypeptide with alpha-1,3-fucosyltransferase activity and in which the sequence of the corresponding nucleic acid is operably linked to the control sequences. Appication of a polynucleotide encoding a polypeptide with alpha-1,3-fucosyltransferase activity to produce a fucosylated oligosaccharide is presented, which is performed by heterologous or homologous expression of a polynucleotide encoding an alpha-1,3-fucosyltransferase. A method for preparation of fucosylated oligosaccharides using the above bacterial host cell is provided.
EFFECT: increased amount of fucosylated oligosaccharides, in particular 3-fucosyllactose, when using lactose as a substrate.
10 cl, 9 dwg, 1 tbl, 1 ex
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