METHODS AND MEANS FOR VEGETABLE GENOME MODIFICATION IN NUCLEOTIDE SEQUENCE, WIDELY USED IN PLANT GENOMIC ENGINEERING Russian patent published in 2017 - IPC C12N15/82

Abstract RU 2639512 C2

FIELD: biotechnology.

SUBSTANCE: invention relates to a method for introduction of a foreign DNA molecule into a pre-determined site in the genome of a transgenic plant cell by inducing double-stranded DNA disruption by administration of a non-naturally occurring single-chain meganuclease or a pair of non-naturally occurring meganucleases, as well as application of the above method to produce a plant cell or a plant. Application of a non-naturally occurring meganuclease or a pair of non-naturally occurring meganucleases to introduce a rupture of double-stranded DNA into the coding region of bar in the transgenic plant cell genome is also disclosed.

EFFECT: invention allows to effectively introduce a foreign DNA molecule into the plant cell genome.

19 cl, 5 dwg, 1 tbl, 5 ex

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RU 2 639 512 C2

Authors

D`Allyuen Ketlin

Dates

2017-12-21—Published

2011-06-07—Filed