FIELD: biochemistry.
SUBSTANCE: invention relates to the field of biochemistry, in particular, to a method for detecting a donor DNA polynucleotide insertion at the targeted genomic locus DAS-59132 of a maize plant. Above method comprises: amplification of a sample of genomic DNA containing the target genomic locus using a first plurality of oligonucleotides SEQ ID NO: 1–5, to obtain the first amplicon containing the target genomic locus, and detecting the presence or absence of the first amplicon, with the absence of the first amplicon indicating the presence of a donor DNA polynucleotide in the target genomic locus.
EFFECT: invention allows to determine the presence of an exogenous donor polynucleotide DNA in the target genomic locus DAS-59132 with high efficiency.
8 cl, 12 dwg, 7 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RAPID TARGETING ANALYSIS IN CROPS FOR DETERMINING DONOR INSERTION | 2014 |
|
RU2668819C2 |
| METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETING OF TRANSGENES | 2013 |
|
RU2650819C2 |
| FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2656158C2 |
| FAD3 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2665811C2 |
| FLUORESCENCE ACTIVATED CELL SORTING (FACS) ENRICHMENT TO GENERATE PLANTS | 2013 |
|
RU2679510C2 |
| UNIVERSAL DONOR SYSTEM FOR DIRECTED ACTION ON GENES | 2014 |
|
RU2687369C2 |
| CONSTRUCTED BY ENGINEERING TECHNIQUES PLATFORM (ETIP) FOR TRANSGENE EMBEDDING FOR GENETIC TARGETING AND TRAIT STACKING | 2013 |
|
RU2666916C2 |
| FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS | 2013 |
|
RU2656159C2 |
| OPTIMAL SOYA LOCI | 2014 |
|
RU2719150C2 |
| TARGETED MODIFICATION OF MALATE DEHYDROGENASE | 2013 |
|
RU2658437C2 |
