FIELD: medicine.
SUBSTANCE: invention relates to methods for determination of antibodies to the virus of type 1, 3 poliomyelitis in the serum, where a killed poliomyelitis virus antigen contained in an inactivated polio vaccine and hemolytic serum are added to the serum in modified complement binding reaction, the samples are maintained, then quantification of oxidase enzymes from hemolysed erythrocytes is performed using tetramethylbenzidine. And the presence of antibodies to polioviruses of types 1 and 3 is determined by the degree of erythrocytes lysis spectrophotometrically by the colour index.
EFFECT: invention provides a possibility of method implementation in any hospital equipped with an ELISA laboratory, preventing the development of outbreaks of poliomyelitis among the population.
2 ex, 2 tbl
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