MICROBIOLOGICAL METHOD OF OBTAINING 17ALPHA-HYDROCORTIZONE ACETATE FROM 17ALPHA,21-CORTEXOLONE DIACETATE Russian patent published in 2018 - IPC C12P33/08 C07J1/00 C12R1/645 

FIELD: biotechnology.

SUBSTANCE: transformation 17α,21-cortexolone diacetate is carried out with the washed from the nutrient medium daily mycelium of the Curvularia lunata VKM F-645 strain of the second generation in 0.05 M potassium phosphate buffer pH 6.0 with the addition of 1% v/v. oil solution of retinol acetate and 0.5% v/v. PEG 400. The mycelium of Curvularia lunata VKM F-645 strain is taken in an amount of 30-35 g/L, based on the weight of dry biomass. The steroid substrate is introduced into the transformation buffer as a hot solution of dimethylformamide (DMF) with a final solvent concentration of 1.5% v/v. followed by incubation and allocation by extraction or distillation of the solvent and crystallization.

EFFECT: increased efficiency.

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