FIELD: biotechnology.
SUBSTANCE: line of mesenchymal stem cells (hb-MSC) for therapeutic use, deposited in the Russian National Collection of Industrial Microorganisms (VKPM) under accession number N-154 is described. At that, the line has at least one of the following characteristics: when plating 0.7×106 cells into 75 cm2 bottle and culturing for at least 96 hours: (a) the cells produce at least 4.5 mM of lactate within 24 hours after medium replacement; (b) the cells produce at least 150 pg/ml of GRO/KC for 24 hours after medium replacement; (c) the cells produce less than 250 pg/ml of OPG 24 hours after medium replacement; or (d) the cells produce less than 80 pg/ml of TGF-β3 24 hours after medium replacement. Also, conditioned media for therapeutic use, obtained by culturing the above-mentioned line, and application of these media are described.
EFFECT: invention expands the arsenal of therapeutic agents.
62 cl, 7 dwg, 3 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| STEM CELL MATERIAL AND METHOD FOR PRODUCTION THEREOF | 2015 |
|
RU2785588C2 |
| STEM CELL MATERIAL, COMPOSITIONS AND METHODS OF USE | 2016 |
|
RU2708329C2 |
| COMPOSITION FOR SKIN AND MUCOUS MEMBRANES REGENERATION | 2016 |
|
RU2621867C1 |
| HUMAN UTERINE CERVICAL STEM CELL POPULATION AND USES THEREOF | 2014 |
|
RU2679500C2 |
| SKIN AND MUCOSA CELL RENEWAL COMPOSITION | 2010 |
|
RU2455354C1 |
| TREATING PERIPHERAL VASCULAR DISEASES BY MEANS OF UMBILICAL CORD TISSUE CELLS | 2011 |
|
RU2576836C2 |
| MESENCHYMAL STROMAL CELLS FOR TREATING SEPSIS | 2015 |
|
RU2715866C2 |
| METHOD FOR SCREENING REGENERATION-PROMOTING NEURONAL CELLS HAVING NEURONAL REGENERATIVE ACTIVITY | 2022 |
|
RU2819367C1 |
| INDUCTION MEDIUM AND METHODS OF STEM CELL CULTIVATION AND THERAPY | 2015 |
|
RU2717983C2 |
| COMPOSITION FOR PREVENTIVE OR THERAPEUTIC TREATMENT OF CONDITIONS ASSOCIATED WITH CHANGING AMOUNT OF HAIR | 2018 |
|
RU2716159C2 |
