METHOD OF COLLECTION AND PRIMARY SOWING OF NASAL LAVAGE FLUID FROM PATIENTS WITH CYSTIC FIBROSIS FOR MICROBIOLOGICAL EXAMINATION Russian patent published in 2018 - IPC G01N33/487 

FIELD: medicine.

SUBSTANCE: invention relates to medicine and can be used for the collection and initial seeding of nasal lavage fluid for patients with cystic fibrosis for microbiological examination. To do this, the nasal cavity is washed with a sterile 1.5–2 % NaCl solution with the help of an individual nasal shower, followed by the collection of freely flowing lavage fluid into a sterile, disposable container. Collected material is transported to the laboratory no later than 2 hours after collection and vortexed. Sowing 200 mcl of collected material is performed by a sterile swab on 5 culture media, including 5 % blood agar, chocolate agar, universal chromogenic medium, selective medium for Burkholderia cepacia (OFPBL-agar), as well as Saburoi agar using “lawn” seeding techniques. Cups with 5 % blood agar, a universal chromogenic medium are incubated in a thermostat at a temperature of 37 °C for 24–48 hours with daily viewing of crops. Cups with chocolate agar are incubated at 35 °C in an atmosphere of 5–7 % CO₂ for 24–48 hours with daily viewing of crops. Seeded cups with selective medium for Burkholderia cepacia (OFPBL agar) and Saburo agar are incubated for 24–48 hours at 37 °C further incubated up to 14 days at room temperature with daily viewing of crops, their subsequent identification and recalculation of CFU per 1 ml of lavage fluid.

EFFECT: invention provides an adequate production of biomaterial from nasal lavage fluid in patients with cystic fibrosis, followed by a qualitative and quantitative assessment of the patient's nasal sinus microbiocenosis.

1 cl, 1 ex