FIELD: biotechnology.
SUBSTANCE: invention relates to the biotechnology. Described is class 2 CRISPR system comprising: (i) a single polynucleotide comprising (a) a targeted region containing deoxyribonucleic acid (DNA) or ribonucleic acid (RNA); and (b) an activating region adjacent to said targeting region containing DNA; and wherein said activating region comprises a stem and a vaginal discharge and is capable of reacting with the Cas9 protein; and (ii) Cas9. Disclosed is a corresponding single polynucleotide. Described is a method of modifying a target nucleic acid molecule in an organism. Described is a method of modulating transcription of at least one gene in a molecule of a target nucleic acid in an organism. Also presented is a kit of two polynucleotides CRISPR class 2.
EFFECT: invention extends the range of products used for genome editing.
132 cl, 14 dwg, 18 tbl, 13 ex
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