CRISPR-CAS SYSTEMS AND METHODS OF CHANGING EXPRESSION OF GENE PRODUCTS Russian patent published in 2019 - IPC C12N15/63 

FIELD: biotechnology.

SUBSTANCE: described is a constructed non-occurring vector system comprising one or more vectors containing a) a first regulatory element operably linked to a nucleotide sequence encoding one or more CRISPR-Cas system RNAs which are capable of hybridising with target sequences in genomic loci of DNA molecules encoding one or more gene products, b) a second regulatory element operably linked to a nucleotide sequence encoding a Cas protein, where the Cas protein is a Cas9 protein and contains one or more NLS, where components (a) and (b) are in the same or different vectors of the system, whereby RNA guides target the genomic loci of DNA molecules encoding one or more gene products in a eukaryotic cell, and Cas protein is capable of splitting genome loci of DNA molecules encoding one or more gene products, as a result of which expression of one or more gene products changes in a eukaryotic cell; and where Cas protein and RNA guides are not found together in vivo. Also described is a structured programmable non-naturally occurring CRISPR-Cas system containing a Cas protein, where the Cas protein is a Cas9 protein and contains one or more NLS, and one or more RNA guide which are capable of targeting genomic loci of DNA molecules encoding one or more gene products in a eukaryotic cell, and Cas protein is capable of splitting genome loci of DNA molecules encoding one or more gene products, as a result of which expression of one or more gene products changes in a eukaryotic cell; and where Cas protein and RNA guides are not found together in vivo. Disclosed are methods of using these systems.

EFFECT: invention extends the range of genome editing tools.

28 cl, 22 dwg, 4 tbl, 7 ex