FIELD: biotechnology.
SUBSTANCE: group of inventions refers to a thermophilic bacterial cell producing (S)-lactic acid obtained genetically by engineering and a process for producing enantiomerically pure (S)-lactic acid. Thermophilic bacterial cell obtained by genetically engineering is proposed, which is an optional anaerobic, gram-positive and producing (S)-lactic acid cell belonging to the Geobacillus genus, in which the endogenous gene mgsA of methyl glyoxal synthase is inactivated or deleted. Method for producing enantiomerically pure (S)-lactic acid, comprising cultivating said cell using a suitable fermentable carbonaceous source of nutrition and isolating (S)-lactic acid, is also provided.
EFFECT: group of inventions provides the enantiomerically pure (S)-lactic acid with a purity of at least 99,7 %.
27 cl, 5 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| VECTOR FOR ACETATE KINASE GENE KNOCKOUT IN Clostridium thermocellum, HOST CELL, GENETICALLY MODIFIED MICROORGANISM Clostridium thermocellum, METHOD OF PRODUCING SAID MICROORGANISM AND METHOD OF CONVERTING LIGNOCELLULOSE BIOMASS TO ETHANOL | 2008 |
|
RU2541785C2 |
| ESCHERICHIA COLI BACTERIUM FOR MAKING 1,2-PROPANEDIOL, METHOD FOR MAKING THEREOF, METHOD FOR MAKING 1,2-PROPANEDIOL | 2005 |
|
RU2407793C2 |
| STRAIN OF BACTERIA Geobacillus stearothermophilus - PRODUCER OF BIOETHANOL | 2013 |
|
RU2534880C1 |
| STRAIN OF BACTERIA ESCHERICHIA COLI-SUCCINIC ACID PRODUCER (VERSIONS) AND METHOD FOR PREPARING SUCCINIC ACID USING THIS STRAIN | 2015 |
|
RU2603004C1 |
| STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF FUMARIC ACID AND METHOD OF OBTAINING FUMARIC ACID USING THIS STRAIN | 2014 |
|
RU2573936C1 |
| METHOD OF OBTAINING L-AMINO ACIDS USING BACTERIA OF FAMILY ENTEROBACTERIACEAE | 2007 |
|
RU2364628C2 |
| RECOMBINANT BACTERIAL STRAIN Escherichia coli THAT IS SUCCINIC ACID PRODUCER (VERSIONS) AND METHOD FOR PREPARING SUCCINIC ACID WITH USING THIS STRAIN | 2012 |
|
RU2528056C2 |
| METHOD OF TREATING CELLULOSE MATERIAL AND ENZYMES USED THEREIN | 2006 |
|
RU2458128C2 |
| METHOD OF OBTAINING 2,4-DIHYDROXYBUTYRIC ACID | 2011 |
|
RU2626531C2 |
| RECOMBINANT MICROORGANISM FOR IMPROVED PRODUCTION OF CHEMICAL PRODUCTS OF FINE ORGANIC SYNTHESIS | 2014 |
|
RU2708312C1 |
