METHOD OF CULTURING MAMMALIAN CELLS AND OBTAINING POLYPEPTIDES USING CELL CULTURE COMPOSITION Russian patent published in 2019 - IPC C12N5/02 C12P21/00 

FIELD: biotechnology.

SUBSTANCE: group of inventions relates to biotechnology, specifically to methods of using a composition of cell culture media for growing cells and/or protein production. Disclosed are a method for culturing mammalian cells and a method for producing a polypeptide comprising a step of contacting cells with a basal medium comprising from about 300 mg/l to about 1,200 mg/l cystine; from about 0.05 mg/l to about 1.0 mg/l vitamin B2; from about 0.05 mg/l to about 10.0 mg/l vitamin B6; from about 0.05 mg/l to about 12.0 mg/l vitamin B9; from about 0.05 mg/l to about 2.5 mg/l vitamin B12; from about 2 mcM to about 80 mcM ferric citrate.

EFFECT: proposed inventions provide the resulting protein-based pharmaceutical products with reduced colour intensity, while maintaining the desired concentration of the protein-based pharmaceutical product, for example, ≥100 mg / ml or ≥150 mg / ml, and may be used, for example, for the production of antibodies for subcutaneous injection.

36 cl, 15 dwg, 11 tbl, 7 ex