FIELD: physics.
SUBSTANCE: invention can be used for measuring/determining analyte concentration in fluid samples, for example whole blood. Proposed is a method of determining analyte concentration using a reduction-oxidation reaction in an electrochemical cell that has at least two electrodes, one of which is a working electrode, wherein at least one electrode being exposed to at least one redox mediator. Method comprises at least one cycle of pulses, each cycle having at least a first potential and a second potential. Method comprises the following steps: applying a first potential to initiate an accumulation phase that forces accumulation of a mediator concentration gradient at or close to the working electrode with a concentration that decreases towards the bulk solution, applying a second potential to initiate a measurement phase and deplete the established mediator concentration gradient at the working electrode; measuring current associated with the second potential of each cycle; and using the measured current to determine analyte concentration.
EFFECT: invention provides the possibility of mitigating the effects of diffusion interfering factors (DIF), especially haematocrit (Hct).
27 cl, 5 dwg
