FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. Method for purification of neutral human milk oligosaccharides (HMO), HMO concentrate and use of HMO concentrate are disclosed. Method includes a combination of processing steps in cation- and anion-exchange devices for fermentation broth, comprising HMO obtained by microbiological fermentation of a recombinant microorganism, with a subsequent step of nanofiltration and/or electrodialysis and, optionally, a step for spraying drying thereof. HMO concentrate is obtained using said method. Concentrate is used in the prevention or treatment of gastrointestinal disorders.
EFFECT: inventions provide efficient purification of large quantities of neutral HMO.
18 cl, 3 dwg, 2 ex
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