METHOD OF PRODUCING NIOSOMAL FORM OF CEFOTAXIME Russian patent published in 2019 - IPC A61K31/429 A61K47/34 

FIELD: technological processes.

SUBSTANCE: invention relates to a method of producing niosomal form of cefatoxime by reversed-phase distillation, comprising: chloroform sorbital monostearate (Span 60) solution, cholesterol, polyethylene glycol-4000 and dicetyl phosphate in molar ratio of 60:34:5:1 respectively (38 mmol of components per 50 ml of chloroform) are mixed with a solution of cefotaxime (3 mg/ml) in 0.01 M phosphate-salt buffer pH 7.4 in ratio of organic and aqueous phases of 5:1, then the mixture is exposed to an ultrasonic disintegrator for 5 minutes, amplitude of 7.5 mcm, frequency of 20 kHz, emulsion is moved into a round bottom flask with a teflon mixer and the chloroform is distilled on a rotary evaporator for 20 minutes at pressure of 0.175 bar, temperature (26±1) °C and 150 rpm, then 25 minutes at pressure of 0.175 bar, temperature (55±1) °C, 200 rpm, then, 20 % of initial volume of aqueous phase is added to mixture and distillation is continued for 45 minutes at pressure of 0.175 bar, temperature (26±1) °C and 140 rpm, the preparation is transferred into clean utensils and left at (20±5) °C for 12 hours.

EFFECT: method provides niosomes with encapsulated cefatoxime with high efficacy of activating substance (63,7 %) and can be used for microencapsulation of antibacterial preparations in niosomes.

1 cl, 5 ex