SCREENING METHOD FOR HIGH PROBABILITY OF PRESENCE OF CHRONIC INFLAMMATION ASSOCIATED WITH BRONCHO-OBSTRUCTIVE SYNDROME, IN BRONCHOPULMONARY DISEASES IN CHILDREN Russian patent published in 2019 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: invention refers to paediatrics, pulmonology, clinical laboratory diagnostics, and can be used for screening of high probability of presence of chronic inflammation in broncho-obstructive syndrome in children older than 2 years. That is ensured by scarifying a portion of the epidermis on the palmar side of the forearm to obtain a cell imprint and an exudate of the skin aseptic inflammatory reaction on slides 6, 24 and 32 hours after scarification. In 6 hours from the moment of scarification, the number of monocytes and lymphocytes in percentage of total leukocyte count and spreading coefficient are determined. After 24 hours, lymphocyte count is determined as a percentage of total leukocyte count, leukocyte count with autolysis as a percentage of total leukocyte count (autolysis index), maximum number of fibres in the field of vision. In 32 hours from the moment of scarification spreading coefficient and autolysis index are determined. If the monocyte count is less than 10 %, the lymphocyte is more than 0.9 % and the spreading factor is more than 1.4 after 6 hours from the scarification, the lymphocyte count is less than 1.5 %, the maximum number of fibres is less than 0.7 in the visual field, autolysis index less than 42 % in 24 hours from the moment of scarification, spreading coefficient more than 1.7 and autolysis index more than 49 % in 32 hours is determined high probability of presence of chronic inflammation associated with broncho-obstructive syndrome.

EFFECT: method enables detecting chronic inflammation in broncho-obstructive syndrome in children with bronchopulmonary diseases ensured by objectivity of detecting chronic nature of inflammation with its accessibility.

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