FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and specifically to a recombinant variolovaccine virus (VVV) strain producing apoptosis-inducing oncotoxic NS1 protein of rat parvovirus H-1 and having oncolytic activity on human glioblastoma. VV-NS1-dGF strain is deposited in the State collection of virus infectious agents and rickettsiosis of FBIS State Research Center of Virology and Biotechnology Vector of Federal Service for Supervision of Consumer Rights and Human Welfare, No. V-803. Recombinant strain VV-NS1-dGF is produced on the basis of strain L-IVP of a variolovaccine virus containing deletion of a gene fragment of a viral growth factor, in area of which there is a synthetic NS1 gene of rat parvovirus H-1. NS1 gene is expressed under the control of an early-late synthetic promoter PE/L VVV to produce a protein with a molecular weight of 100 kDa.
EFFECT: invention widens the range of products for developing new-generation drugs for combating oncological diseases.
1 cl, 6 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT STRAIN VV-mNIS-NS1 OF VACCINIA VIRUS PRODUCING mNIS SODIUM IODIDE SYMPORTER OF MICE AND NS1 PROTEIN OF RAT PARVOVIRUS H-1 FOR MALIGNANT TUMOUR THERANOSTICS | 2023 |
|
RU2819082C1 |
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|
RU2604187C1 |
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|
RU2630672C1 |
| RECOMBINANT STRAIN OF VV-GMCSF-S-Lact VACCINIA VIRUS, WITH ONCOLYTIC ACTIVITY, PRODUCING GRANULOCYTOR-MACROPHAGAL COLONY-STIMULATING HUMAN FACTOR AND SECRETED FORM OF LACTAPTIN ONCOTOXIC PROTEIN | 2016 |
|
RU2621861C1 |
| RECOMBINANT PLASMID DNA pGEM-Puro-DS-Apo COMPRISING SYNTHETIC APOPTIN GENE FLANKED BY SEQUENCES OF VACCINIA VIRUS GENOME OF ISLAND C10L-C12L, AND RECOMBINANT STRAIN VVdGF-ApoS24/2 OF VACCINIA VIRUS PRODUCING APOPTIN | 2012 |
|
RU2492238C1 |
| RECOMBINANT ONCOLYTIC STRAIN OF THE VACCINIA VIRUS L-IVP_ONCOB, CONTAINING GENES, ENCODING GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR AND POLYEPITOPE IMMUNOGEN, CONSISTING OF EPITOPES OF ANTIGENS, HYPER EXPRESSING BY TUMOR CELLS IN BREAST CANCER | 2017 |
|
RU2678056C1 |
| RECOMBINANT ONCOLYTIC STRAIN OF L-IVP_ONCOQ VACCINIA VIRUS, CONTAINING GENES ENCODING GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR AND POLYEPITOPE IMMUNOGEN CONSISTING OF ANTIGEN EPITOPES, OVEREXPRESSING TUMOR CELLS IN OVARIAN CANCER | 2017 |
|
RU2678003C1 |
| RECOMBINANT ONCOLYTIC STRAIN OF L-IVP_ONCOM VACCINIA VIRUS, CONTAINING GENES, ENCODING GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTOR AND POLYEPITOPE IMMUNOGEN CONSISTING OF ANTIGEN EPITOPES, OVEREXPRESSING TUMOR CELLS IN MELANOMA | 2017 |
|
RU2678060C1 |
| RECOMBINANT STRAIN VV-GMCSF-S1/3 OF VACCINIA VIRUS, PRODUCING SECRETED GRANULOCYTE-MACROPHAGE COLONY-STIMULATING HUMAN FACTOR | 2013 |
|
RU2565544C2 |
| ANTI-TUMOUR AGENT BASED ON RECOMBINANT VACCINIA VIRUS STRAIN AND METHOD FOR PRODUCTION THEREOF | 2019 |
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RU2730657C1 |
