FIELD: biotechnology.
SUBSTANCE: disclosed is a modified polypeptide having O-acetyl-homoserine-sulfhydrylase activity, where 196th amino acid from the N-terminus of the polypeptide represented by the amino acid sequence SEQ ID NO: 1, valine, is replaced with threonine. Polynucleotide encoding said modified polypeptide is provided. Disclosed is an expression vector containing said polynucleotide. Disclosed is a microorganism of the genus Escherichia, which produces O-acetyl-homoserine-sulfhydrylase, which expresses said modified polypeptide having O-acetyl-homoserine-sulfhydrylase activity, or contains said expression vector. Disclosed is a method for producing methionine involving reacting said modified polypeptide or microorganism producing said modified polypeptide or product of its cultivation with O-acetylhomoserine and methylmercaptan.
EFFECT: group of inventions enables to increase production of methionine using said modified polypeptide compared to the wild type of said polypeptide.
7 cl, 1 dwg, 4 tbl, 3 ex
| Title |
Year |
Author |
Number |
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2016 |
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- Kim So Young
|
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| NOVEL O-ACETYLHOMOSERINE-SULPHHYDRILASE OR ITS MUTANT PROTEIN AND METHOD FOR CONVERSION ONTO METHIONINE WITH THEREOF APPLICATION |
2011 |
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- Son Sung Kvang
- Kheo In Kiung
- Li Khan Dzin'
- Kim Dzu Eun
- Kim Khiun Akh
- Bae Dzee Jeon
- Na Kvang Kho
|
RU2573928C2 |
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2020 |
- Choi Sol
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- Lee Han Hyoung
|
RU2827731C1 |
| NEW EMBODIMENT OF ASPARTOKINASE AND METHOD OF PRODUCING L-AMINO ACID USING EMBODIMENT THEREOF |
2018 |
- Kim Hyung Joon
- Kim Hyo Jin
- Bae Hyun Won
- Kim Hyun Ah
- Seo Chang Il
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- Chang Jin Sook
|
RU2730025C1 |
| MICROORGANISMS PRODUCING O-ACETYL HOMOSERINE AND METHOD FOR O-ACETYL HOMOSERINE PRODUCTION USING THIS MICROORGANISM |
2015 |
- Kim Hyun Ah
- Seo Ju Hee
- Shin Yong Uk
- Kim So Young
- Kim Sang Kyoum
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- Son Sung Kwang
- Yoo Hye Ryun
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|
RU2614253C1 |
| VERSION OF O-PHOSPHOSERINE EXPORTING PROTEIN, AND METHOD OF PRODUCING O-PHOSPHOSERINE, CYSTEINE AND DERIVATIVES THEREOF USING SAME |
2021 |
- Park Hye Min
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|
RU2821317C1 |
| METHOD OF PRODUCING SULPHUR-CONTAINING AMINO ACID OR DERIVATIVE THEREOF |
2020 |
- Choi Sol
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|
RU2814546C2 |
| MODIFIED HOMOSERINE DEHYDROGENASE AND METHOD FOR PRODUCING HOMOSERINE OR L-AMINO ACID OF HOMOSERINE ORIGIN USING SUCH HOMOSERINE DEHYDROGENASE |
2019 |
- Kim Hyo Jin
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- Lim Sang Jo
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- Kim Hyoung Joon
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- Lee Seung Bin
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|
RU2733426C1 |
| VARIANT OF INNER MEMBRANE PROTEIN AND METHOD FOR OBTAINING TARGET PRODUCT USING IT |
2020 |
- Kim Seo Yun
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- Baek Min Ji
|
RU2804941C1 |
| MODIFIED POLYPEPTIDE HAVING HOMOSERINE ACETYLTRANSFERASE ACTIVITY AND MICROORGANISM EXPRESSING IT |
2011 |
- Kim, So Joung
- Sin, Jong Juk
- Seo, Chang Il
- Kheo, In Kiung
- Kim, Dzu Eun
- Kim, Khiun Akh
- Li, Khan Dzin'
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- Son, Sung Kvang
|
RU2557411C2 |
