METHODS FOR PRODUCING FRAME-LIPIDS Russian patent published in 2019 - IPC C12P19/44 A01N43/16 C12N15/52 C12N9/02 C12N9/10 C12N9/14 

FIELD: biotechnology.

SUBSTANCE: disclosed is a method of producing at least one ramolipid, comprising: bringing the recombinant cell into contact with a medium containing a carbon source; and culturing the cell in suitable conditions to obtain a ramolipid from a carbon source using a cell, where the recombinant cell is genetically modified such that compared to the wild-type cell, the cell is characterized by high activity of enzymes E₁, and E₂, and E₃, and oxidoreductase of alkB type or high activity of enzymes E₁, and E₂, and alkB-type oxidoreductase, where enzyme E₁ is α/β-hydrolase, enzyme E₂ is rhamnosyl transferase I and enzyme E₃ is rhamnosyl transferase II and where carbon source is C₄-molecule, where C₄-molecule is selected from a group comprising butane, 1-butanol, 2-butanol, 1-butanal, butanone, butyric acid and combinations thereof. Disclosed is a cell capable of forming at least one ramolipid C₄-molecule intended for implementation of said method, where the cell is a bacterium and genetically modified such that compared to a wild-type cell, the cell is characterized by high activity of enzymes E₁, and E₂, and E₃, and oxidoreductase of alkB type or high activity of enzymes E₁, and E₂, and alkB-type oxidoreductase, where C₄-molecule is selected from a group comprising butane, 1-butanol, 2-butanol, 1-butanal, butanone, butyric acid and combinations thereof.

EFFECT: group of inventions enables to obtain the rhinolipids (mono-nornosyl-lipids and/or dianmarnosyl-lipids) from a carbon source which is said C₄-molecule, with reduced amount of obtained undesirable by-products and intermediate products, such as dimers of fatty β-hydroxy acids.

12 cl, 1 dwg, 2 tbl, 4 ex