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2 792 274

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IPC

A61K31/573(2006-01-01)

G01N30/02(2006-01-01)

G01N30/34(2006-01-01)

G01N30/74(2006-01-01)

G01N33/48(2006-01-01)

(21) (22)

Application

2022133997, 2022-12-23

(24)

Start date

2022-12-23

(22)

Actual filing date

2022-12-23

(45)

Published

2023-03-21

(72)

Inventor

Kulikov Oleg AleksandrovichShlyapkina Vasilisa IgorevnaZaborovskij Andrej VladimirovichYunina Dina VladimirovnaTararina Larisa AnatolevnaAgeev Valentin PavlovichZharkov Mikhail NikolaevichDadaeva Izabella MikhajlovnaKheladze Natalya VarlamovnaPyataev Nikolaj Anatolevich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniya Gosudarstvennyj Mediko-Stomatologicheskij Universitet Imeni A.I. Evdokimova" Ministerstva Zdravookhraneniya Rossijskoj Federatsii Vo Mgmsu Im. A.I. Evdokimova Minzdrava Rossii)

METHOD FOR QUANTITATIVE DETERMINATION OF DEXAMETHASONE IN BIOLOGICAL MEDIA USING HPLC WITH ULTRAVIOLET DETECTION Russian patent published in 2023 - IPC A61K31/573 G01N30/02 G01N30/34 G01N30/74 G01N33/48

Abstract RU 2792274 C1

FIELD: analytical; pharmaceutical chemistry.

SUBSTANCE: invention relates to a method for the quantitative determination of dexamethasone in biological media. A method for the quantitative determination of dexamethasone in biological media using HPLC with ultraviolet detection is proposed, according to which the extraction of dexamethasone from biosamples is carried out by liquid-liquid extraction with acetone in an acidic medium, a mixture of methanol and phosphate buffer in a ratio of 50:50 is used as the mobile phase, the stationary phase represented by Kromasil 100 С18, 250 column×4.6 mm, 5 µm, detection is carried out at 241 nm, and atenolol is used as an internal standard.

EFFECT: above method is a highly sensitive for the determination of dexamethasone in biological media using HPLC-UV.

1 cl, 7 dwg, 2 ex

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RU 2 792 274 C1

Authors

Kulikov Oleg Aleksandrovich

Shlyapkina Vasilisa Igorevna

Zaborovskij Andrej Vladimirovich

Yunina Dina Vladimirovna

Tararina Larisa Anatolevna

Ageev Valentin Pavlovich

Zharkov Mikhail Nikolaevich

Dadaeva Izabella Mikhajlovna

Kheladze Natalya Varlamovna

Pyataev Nikolaj Anatolevich

Dates

2023-03-21—Published

2022-12-23—Filed