FIELD: chemistry.
SUBSTANCE: invention relates to analytical chemistry. Disclosed is a method for quantitative determination of doxorubicin in biological samples, including separation of doxorubicin from biological samples by liquid organic phase extraction, specifically a mixture of methanol, 37% HCl and dimethyl sulphoxide in a volume ratio of 5:5:7, identifying doxorubicin by recording its characteristic fluorescence spectrum and obtaining doxorubicin fluorescence intensities from biological samples on λ=595 nm with excitation with light with λ=495 nm to determine the concentration of doxorubicin in a biological sample, which is blood plasma or homogenates of organs or tissues.
EFFECT: invention provides a high degree of extraction of the preparation, a high lower limit of detection and quantification, the required linearity, accuracy and precision.
1 cl, 17 dwg, 3 tbl
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