METHOD FOR PRODUCING PARVOVIRUS CHARACTERIZED BY HIGH INFECTIVITY TITRE AND HIGH PURITY Russian patent published in 2019 - IPC C12N7/00 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology and specifically to a method of producing parvovirus derived from an unconjugated cell culture supernatant. Method comprises (a) a pre-calculation step every 24 hours of a time-dependent change in cell density in a culture substrate, when host cells are infected with parvovirus for each cell density (A) when infected with a virus; (b) a step for determining, based on the time-dependent change in cell density, calculated at step (a), (b1), the time (T_max) from infection to time of peak time-dependent change in cell density, (b2) cell density (B_max) at T_max and A1, which is A, which satisfies following equation (1) B_max/A1>1.2, (b3) maximum (A_max) cell density A1 when infected with virus and (b4) A2, which satisfies following equation (2) A_max≥A₂≥A_max/10; (c) a stage of inoculation of seed parvovirus into a culture substrate containing host cells having cell density A2, wherein the infecting virus defined in (b4) of step (b) and the serum medium give a multiplicity of infection (MOI) of 0.001 to 0.1; (d) a cultivation stage of a cultured product containing host cells and parvovirus obtained at step (c) for a period of time T_max or more to less than (T_max+48) hours, where T_max is defined in (b1) of step (b); (e) a step for replacing the culture supernatant obtained in step (d) with a serum-free medium and culturing for 12 hours or more; and (f) a step for collecting a parvovirus-containing culture supernatant produced by culturing at step (e); where at step (b), when A satisfying equation (1) is absent, steps (a) and (b) are repeated by using another density of cells A when infected with virus.

EFFECT: invention enables to obtain parvovirus with high infectivity titre for use in evaluating viral safety of pharmaceutical or biological products or when producing vaccines.

9 cl, 10 tbl, 4 ex