FIELD: biotechnologies.
SUBSTANCE: invention relates to the biotechnology. A method for producing botulinum toxin involves: (a) treating the culture of the botulinum toxin-producing strain with an acid to form a residue containing botulinum toxin, followed by neutralizing the pH of the precipitate, (b) preparing a solution for anion-exchange chromatography containing a buffer, using membrane filtration techniques and membrane chromatography and (c) purifying botulinum toxin by anion-exchange chromatography, wherein DNase and RNase are not used in said method.
EFFECT: invention enables to purify botulinum toxin from DNA and RNA in the absence of the enzymatic treatment step.
27 cl, 7 dwg, 2 ex
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